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plasma adiponectin 22-adpms-e01  (ALPCO)


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    ALPCO plasma adiponectin 22-adpms-e01
    Construction and validation of adipose-specific conditional Angptl8 KO mice (A) Diagram of the Angptl8 gene with targeted ΔNeo allele, and the disrupted A8 allele. LoxP sites were placed upstream of exon 1 and downstream of exon 4. A vNEO cassette was inserted after exon 4. Mice with the targeted ΔNeo allele (Fl/Fl) were crossed with mice expressing Cre under the control of the <t>adiponectin</t> promoter to inactivate A8 in adipocytes (As-A8−/− mice). NDEL1 and NDEL2 primers used for genotyping are indicated. (B) The presence of the WT, Adipo-Cre transgene or the floxed A8 alleles was evaluated in DNA obtained from ear tissue biopsies using the primers as described in Methods. Specific amplification of a 686 bp DNA fragment corresponds to the floxed- A8 allele while a 569 bp DNA fragment corresponds to the WT A8 allele, the presence of a 600 bp DNA fragment denotes the Adipo-Cre transgene, as verified by genomic PCR. (C) Angptl8 mRNA expression in VAT, SAT, BAT and Liver tissue ( n = 4) were measured in 11-week-old male Cre and AT-A8-KO mice, 3 weeks after TMX treatment. Values are means ± SEM. Multiple unpaired t-test for multiple comparison were performed with Holm-Šídák correction using Graphpad Prism 10. (D) Angptl8 protein expression in collagenase treated mature adipocytes from VAT and SAT tissue in chow diet fed Cre and KO mice (n = 3–4); MM, Pre-stained Molecular weight markers. Quantification are shown in <xref ref-type=Figure S2 B. (E) Body weight ( n = 15), (F) lean mass and fat mass, ( n = 15) and (G) fasted glycemia ( n = 5) in chow diet fed 11-week-old Cre and AT-A8-KO mice. Data are means ± SEM. Groups were compared using unpaired t-test. For Cre vs. KO: ∗ p ≤ 0.033, ∗∗ p ≤ 0.002, ∗∗∗ p < 0.001. VAT, visceral adipose tissue; SAT, subcutaneous adipose tissue, BAT, brown adipose tissue. " width="250" height="auto" />
    Plasma Adiponectin 22 Adpms E01, supplied by ALPCO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasma adiponectin 22-adpms-e01/product/ALPCO
    Average 90 stars, based on 1 article reviews
    plasma adiponectin 22-adpms-e01 - by Bioz Stars, 2026-03
    90/100 stars

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    1) Product Images from "Adipocyte Angptl8 deletion improves glucose and energy metabolism and obesity associated inflammation in mice"

    Article Title: Adipocyte Angptl8 deletion improves glucose and energy metabolism and obesity associated inflammation in mice

    Journal: iScience

    doi: 10.1016/j.isci.2024.111292

    Construction and validation of adipose-specific conditional Angptl8 KO mice (A) Diagram of the Angptl8 gene with targeted ΔNeo allele, and the disrupted A8 allele. LoxP sites were placed upstream of exon 1 and downstream of exon 4. A vNEO cassette was inserted after exon 4. Mice with the targeted ΔNeo allele (Fl/Fl) were crossed with mice expressing Cre under the control of the adiponectin promoter to inactivate A8 in adipocytes (As-A8−/− mice). NDEL1 and NDEL2 primers used for genotyping are indicated. (B) The presence of the WT, Adipo-Cre transgene or the floxed A8 alleles was evaluated in DNA obtained from ear tissue biopsies using the primers as described in Methods. Specific amplification of a 686 bp DNA fragment corresponds to the floxed- A8 allele while a 569 bp DNA fragment corresponds to the WT A8 allele, the presence of a 600 bp DNA fragment denotes the Adipo-Cre transgene, as verified by genomic PCR. (C) Angptl8 mRNA expression in VAT, SAT, BAT and Liver tissue ( n = 4) were measured in 11-week-old male Cre and AT-A8-KO mice, 3 weeks after TMX treatment. Values are means ± SEM. Multiple unpaired t-test for multiple comparison were performed with Holm-Šídák correction using Graphpad Prism 10. (D) Angptl8 protein expression in collagenase treated mature adipocytes from VAT and SAT tissue in chow diet fed Cre and KO mice (n = 3–4); MM, Pre-stained Molecular weight markers. Quantification are shown in <xref ref-type=Figure S2 B. (E) Body weight ( n = 15), (F) lean mass and fat mass, ( n = 15) and (G) fasted glycemia ( n = 5) in chow diet fed 11-week-old Cre and AT-A8-KO mice. Data are means ± SEM. Groups were compared using unpaired t-test. For Cre vs. KO: ∗ p ≤ 0.033, ∗∗ p ≤ 0.002, ∗∗∗ p < 0.001. VAT, visceral adipose tissue; SAT, subcutaneous adipose tissue, BAT, brown adipose tissue. " title="... mice expressing Cre under the control of the adiponectin promoter to inactivate A8 in adipocytes (As-A8−/− mice). ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Construction and validation of adipose-specific conditional Angptl8 KO mice (A) Diagram of the Angptl8 gene with targeted ΔNeo allele, and the disrupted A8 allele. LoxP sites were placed upstream of exon 1 and downstream of exon 4. A vNEO cassette was inserted after exon 4. Mice with the targeted ΔNeo allele (Fl/Fl) were crossed with mice expressing Cre under the control of the adiponectin promoter to inactivate A8 in adipocytes (As-A8−/− mice). NDEL1 and NDEL2 primers used for genotyping are indicated. (B) The presence of the WT, Adipo-Cre transgene or the floxed A8 alleles was evaluated in DNA obtained from ear tissue biopsies using the primers as described in Methods. Specific amplification of a 686 bp DNA fragment corresponds to the floxed- A8 allele while a 569 bp DNA fragment corresponds to the WT A8 allele, the presence of a 600 bp DNA fragment denotes the Adipo-Cre transgene, as verified by genomic PCR. (C) Angptl8 mRNA expression in VAT, SAT, BAT and Liver tissue ( n = 4) were measured in 11-week-old male Cre and AT-A8-KO mice, 3 weeks after TMX treatment. Values are means ± SEM. Multiple unpaired t-test for multiple comparison were performed with Holm-Šídák correction using Graphpad Prism 10. (D) Angptl8 protein expression in collagenase treated mature adipocytes from VAT and SAT tissue in chow diet fed Cre and KO mice (n = 3–4); MM, Pre-stained Molecular weight markers. Quantification are shown in Figure S2 B. (E) Body weight ( n = 15), (F) lean mass and fat mass, ( n = 15) and (G) fasted glycemia ( n = 5) in chow diet fed 11-week-old Cre and AT-A8-KO mice. Data are means ± SEM. Groups were compared using unpaired t-test. For Cre vs. KO: ∗ p ≤ 0.033, ∗∗ p ≤ 0.002, ∗∗∗ p < 0.001. VAT, visceral adipose tissue; SAT, subcutaneous adipose tissue, BAT, brown adipose tissue.

    Techniques Used: Biomarker Discovery, Expressing, Control, Amplification, Comparison, Staining, Molecular Weight

    AT-A8-KO Mice on HFHF diet Reduces Inflammatory Markers (A) MAC-2 immunostaining in VAT tissue section from Cre and KO mice on HFHF diet and (B) quantification of crown-like structures (CLS) ( n = 6 mice, 4 fields/tissue section, original magnification ×20; scale bar = 400 μm). Values are means ± SEM and groups were compared using unpaired t-test. ∗∗ p ≤ 0.002. (C) Plasma MCP-1, (D) Leptin and (E) Adiponectin levels in mice under fed state were quantified by ELISA or AlphaLISA (n = 8–10). Values are means ± SEM. Groups were compared using two-way ANOVA test using GraphPad Prism 10. ∗ p ≤ 0.033, ∗∗ p ≤ 0.002, ∗∗∗ p < 0.001.
    Figure Legend Snippet: AT-A8-KO Mice on HFHF diet Reduces Inflammatory Markers (A) MAC-2 immunostaining in VAT tissue section from Cre and KO mice on HFHF diet and (B) quantification of crown-like structures (CLS) ( n = 6 mice, 4 fields/tissue section, original magnification ×20; scale bar = 400 μm). Values are means ± SEM and groups were compared using unpaired t-test. ∗∗ p ≤ 0.002. (C) Plasma MCP-1, (D) Leptin and (E) Adiponectin levels in mice under fed state were quantified by ELISA or AlphaLISA (n = 8–10). Values are means ± SEM. Groups were compared using two-way ANOVA test using GraphPad Prism 10. ∗ p ≤ 0.033, ∗∗ p ≤ 0.002, ∗∗∗ p < 0.001.

    Techniques Used: Immunostaining, Clinical Proteomics, Enzyme-linked Immunosorbent Assay


    Figure Legend Snippet:

    Techniques Used: Recombinant, Enzyme-linked Immunosorbent Assay, Activity Assay, Software



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    plasma adiponectin 22-adpms-e01  (ALPCO)
    90
    ALPCO plasma adiponectin 22-adpms-e01
    Construction and validation of adipose-specific conditional Angptl8 KO mice (A) Diagram of the Angptl8 gene with targeted ΔNeo allele, and the disrupted A8 allele. LoxP sites were placed upstream of exon 1 and downstream of exon 4. A vNEO cassette was inserted after exon 4. Mice with the targeted ΔNeo allele (Fl/Fl) were crossed with mice expressing Cre under the control of the <t>adiponectin</t> promoter to inactivate A8 in adipocytes (As-A8−/− mice). NDEL1 and NDEL2 primers used for genotyping are indicated. (B) The presence of the WT, Adipo-Cre transgene or the floxed A8 alleles was evaluated in DNA obtained from ear tissue biopsies using the primers as described in Methods. Specific amplification of a 686 bp DNA fragment corresponds to the floxed- A8 allele while a 569 bp DNA fragment corresponds to the WT A8 allele, the presence of a 600 bp DNA fragment denotes the Adipo-Cre transgene, as verified by genomic PCR. (C) Angptl8 mRNA expression in VAT, SAT, BAT and Liver tissue ( n = 4) were measured in 11-week-old male Cre and AT-A8-KO mice, 3 weeks after TMX treatment. Values are means ± SEM. Multiple unpaired t-test for multiple comparison were performed with Holm-Šídák correction using Graphpad Prism 10. (D) Angptl8 protein expression in collagenase treated mature adipocytes from VAT and SAT tissue in chow diet fed Cre and KO mice (n = 3–4); MM, Pre-stained Molecular weight markers. Quantification are shown in <xref ref-type=Figure S2 B. (E) Body weight ( n = 15), (F) lean mass and fat mass, ( n = 15) and (G) fasted glycemia ( n = 5) in chow diet fed 11-week-old Cre and AT-A8-KO mice. Data are means ± SEM. Groups were compared using unpaired t-test. For Cre vs. KO: ∗ p ≤ 0.033, ∗∗ p ≤ 0.002, ∗∗∗ p < 0.001. VAT, visceral adipose tissue; SAT, subcutaneous adipose tissue, BAT, brown adipose tissue. " width="250" height="auto" />
    Plasma Adiponectin 22 Adpms E01, supplied by ALPCO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasma adiponectin 22-adpms-e01/product/ALPCO
    Average 90 stars, based on 1 article reviews
    plasma adiponectin 22-adpms-e01 - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Construction and validation of adipose-specific conditional Angptl8 KO mice (A) Diagram of the Angptl8 gene with targeted ΔNeo allele, and the disrupted A8 allele. LoxP sites were placed upstream of exon 1 and downstream of exon 4. A vNEO cassette was inserted after exon 4. Mice with the targeted ΔNeo allele (Fl/Fl) were crossed with mice expressing Cre under the control of the adiponectin promoter to inactivate A8 in adipocytes (As-A8−/− mice). NDEL1 and NDEL2 primers used for genotyping are indicated. (B) The presence of the WT, Adipo-Cre transgene or the floxed A8 alleles was evaluated in DNA obtained from ear tissue biopsies using the primers as described in Methods. Specific amplification of a 686 bp DNA fragment corresponds to the floxed- A8 allele while a 569 bp DNA fragment corresponds to the WT A8 allele, the presence of a 600 bp DNA fragment denotes the Adipo-Cre transgene, as verified by genomic PCR. (C) Angptl8 mRNA expression in VAT, SAT, BAT and Liver tissue ( n = 4) were measured in 11-week-old male Cre and AT-A8-KO mice, 3 weeks after TMX treatment. Values are means ± SEM. Multiple unpaired t-test for multiple comparison were performed with Holm-Šídák correction using Graphpad Prism 10. (D) Angptl8 protein expression in collagenase treated mature adipocytes from VAT and SAT tissue in chow diet fed Cre and KO mice (n = 3–4); MM, Pre-stained Molecular weight markers. Quantification are shown in <xref ref-type=Figure S2 B. (E) Body weight ( n = 15), (F) lean mass and fat mass, ( n = 15) and (G) fasted glycemia ( n = 5) in chow diet fed 11-week-old Cre and AT-A8-KO mice. Data are means ± SEM. Groups were compared using unpaired t-test. For Cre vs. KO: ∗ p ≤ 0.033, ∗∗ p ≤ 0.002, ∗∗∗ p < 0.001. VAT, visceral adipose tissue; SAT, subcutaneous adipose tissue, BAT, brown adipose tissue. " width="100%" height="100%">

    Journal: iScience

    Article Title: Adipocyte Angptl8 deletion improves glucose and energy metabolism and obesity associated inflammation in mice

    doi: 10.1016/j.isci.2024.111292

    Figure Lengend Snippet: Construction and validation of adipose-specific conditional Angptl8 KO mice (A) Diagram of the Angptl8 gene with targeted ΔNeo allele, and the disrupted A8 allele. LoxP sites were placed upstream of exon 1 and downstream of exon 4. A vNEO cassette was inserted after exon 4. Mice with the targeted ΔNeo allele (Fl/Fl) were crossed with mice expressing Cre under the control of the adiponectin promoter to inactivate A8 in adipocytes (As-A8−/− mice). NDEL1 and NDEL2 primers used for genotyping are indicated. (B) The presence of the WT, Adipo-Cre transgene or the floxed A8 alleles was evaluated in DNA obtained from ear tissue biopsies using the primers as described in Methods. Specific amplification of a 686 bp DNA fragment corresponds to the floxed- A8 allele while a 569 bp DNA fragment corresponds to the WT A8 allele, the presence of a 600 bp DNA fragment denotes the Adipo-Cre transgene, as verified by genomic PCR. (C) Angptl8 mRNA expression in VAT, SAT, BAT and Liver tissue ( n = 4) were measured in 11-week-old male Cre and AT-A8-KO mice, 3 weeks after TMX treatment. Values are means ± SEM. Multiple unpaired t-test for multiple comparison were performed with Holm-Šídák correction using Graphpad Prism 10. (D) Angptl8 protein expression in collagenase treated mature adipocytes from VAT and SAT tissue in chow diet fed Cre and KO mice (n = 3–4); MM, Pre-stained Molecular weight markers. Quantification are shown in Figure S2 B. (E) Body weight ( n = 15), (F) lean mass and fat mass, ( n = 15) and (G) fasted glycemia ( n = 5) in chow diet fed 11-week-old Cre and AT-A8-KO mice. Data are means ± SEM. Groups were compared using unpaired t-test. For Cre vs. KO: ∗ p ≤ 0.033, ∗∗ p ≤ 0.002, ∗∗∗ p < 0.001. VAT, visceral adipose tissue; SAT, subcutaneous adipose tissue, BAT, brown adipose tissue.

    Article Snippet: Additionally, plasma insulin (AL204, PerkinElmer, Vaughan, ON, Canada), leptin (AL225, PerkinElmer), FFA (Fujifilm, Lexington, MA), adiponectin (22-ADPMS-E01, Alpco, Salem, NH), and inflammation markers (IL-6, TNFα, MCP-1) were quantified at the Metabolomic Core Facility of CRCHUM using EnVision (PerkinElmer).

    Techniques: Biomarker Discovery, Expressing, Control, Amplification, Comparison, Staining, Molecular Weight

    AT-A8-KO Mice on HFHF diet Reduces Inflammatory Markers (A) MAC-2 immunostaining in VAT tissue section from Cre and KO mice on HFHF diet and (B) quantification of crown-like structures (CLS) ( n = 6 mice, 4 fields/tissue section, original magnification ×20; scale bar = 400 μm). Values are means ± SEM and groups were compared using unpaired t-test. ∗∗ p ≤ 0.002. (C) Plasma MCP-1, (D) Leptin and (E) Adiponectin levels in mice under fed state were quantified by ELISA or AlphaLISA (n = 8–10). Values are means ± SEM. Groups were compared using two-way ANOVA test using GraphPad Prism 10. ∗ p ≤ 0.033, ∗∗ p ≤ 0.002, ∗∗∗ p < 0.001.

    Journal: iScience

    Article Title: Adipocyte Angptl8 deletion improves glucose and energy metabolism and obesity associated inflammation in mice

    doi: 10.1016/j.isci.2024.111292

    Figure Lengend Snippet: AT-A8-KO Mice on HFHF diet Reduces Inflammatory Markers (A) MAC-2 immunostaining in VAT tissue section from Cre and KO mice on HFHF diet and (B) quantification of crown-like structures (CLS) ( n = 6 mice, 4 fields/tissue section, original magnification ×20; scale bar = 400 μm). Values are means ± SEM and groups were compared using unpaired t-test. ∗∗ p ≤ 0.002. (C) Plasma MCP-1, (D) Leptin and (E) Adiponectin levels in mice under fed state were quantified by ELISA or AlphaLISA (n = 8–10). Values are means ± SEM. Groups were compared using two-way ANOVA test using GraphPad Prism 10. ∗ p ≤ 0.033, ∗∗ p ≤ 0.002, ∗∗∗ p < 0.001.

    Article Snippet: Additionally, plasma insulin (AL204, PerkinElmer, Vaughan, ON, Canada), leptin (AL225, PerkinElmer), FFA (Fujifilm, Lexington, MA), adiponectin (22-ADPMS-E01, Alpco, Salem, NH), and inflammation markers (IL-6, TNFα, MCP-1) were quantified at the Metabolomic Core Facility of CRCHUM using EnVision (PerkinElmer).

    Techniques: Immunostaining, Clinical Proteomics, Enzyme-linked Immunosorbent Assay

    Journal: iScience

    Article Title: Adipocyte Angptl8 deletion improves glucose and energy metabolism and obesity associated inflammation in mice

    doi: 10.1016/j.isci.2024.111292

    Figure Lengend Snippet:

    Article Snippet: Additionally, plasma insulin (AL204, PerkinElmer, Vaughan, ON, Canada), leptin (AL225, PerkinElmer), FFA (Fujifilm, Lexington, MA), adiponectin (22-ADPMS-E01, Alpco, Salem, NH), and inflammation markers (IL-6, TNFα, MCP-1) were quantified at the Metabolomic Core Facility of CRCHUM using EnVision (PerkinElmer).

    Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Activity Assay, Software